Relationship between the non-volatile composition and the in-mouth quality in red wines

Póster presentado en la Third Edition of International Conference Series on Wine Active Compounds (WAC2014), celebrada en Borgoña (Francia) del 26 al 28 de marzo de 2014.Peer Reviewe

Recovery of commercial natural bivalve populations in the Carreras River (Huelva, southwestern Iberian Peninsula)

Techniques in marine resources management have been assessed to establish a methodology for the restoration of commercial shellfish beds in the Carreras River (southwestern Iberian Peninsula), considered as extensive semi-cultivations. The following experimental actions or substrate modifications were analysed during the years 2003 and 2004: 1) oxygenation and cleaning of substrate, 2) changing of the substrate by sand addition, and 3) restocking with autochthonous species.Para establecer una metodología aplicable a la recuperación de los yacimientos naturales de moluscos bivalvos de interés comercial en el río Carreras (suroeste de la península Ibérica), se han ensayado técnicas empleadas en la gestión de recursos, considerándolos como semicultivos extensivos. Durante los años 2003 y 2004 se han aplicado diferentes tratamientos: 1) oxigenación y limpieza del sustrato; 2) enmiendas edáficas por adición de áridos; y 3) repoblaciones con especies autóctonas. La adición de áridos ha proporcionado resultados significativos, a diferencia de los tratamientos de oxigenación y limpieza, de escasa incidencia en el aumento del reclutamiento. Las siembras con especies autóctonas han registrado bajos índices de supervivencia y no han supuesto mejoras en las poblaciones.Instituto Español de Oceanografí

Environment of the submillimeter-bright massive starburst HFLS3 at z∼z\sim6.34

We describe the search for Lyman-break galaxies (LBGs) near the sub-millimeter bright starburst galaxy HFLS3 at $z$$=$6.34 and a study on the environment of this massive galaxy during the end of reionization.We performed two independent selections of LBGs on images obtained with the \textit{Gran Telescopio Canarias} (GTC) and the \textit{Hubble Space Telescope} (HST) by combining non-detections in bands blueward of the Lyman-break and color selection. A total of 10 objects fulfilling the LBG selection criteria at $z$$>$5.5 were selected over the 4.54 and 55.5 arcmin$^2$ covered by our HST and GTC images, respectively. The photometric redshift, UV luminosity, and the star-formation rate of these sources were estimated with models of their spectral energy distribution. These $z$$\sim$6 candidates have physical properties and number densities in agreement with previous results. The UV luminosity function at $z$$\sim$6 and a Voronoi tessellation analysis of this field shows no strong evidence for an overdensity of relatively bright objects (m$_{F105W}$$<$25.9) associated with \textit{HFLS3}. However, the over-density parameter deduced from this field and the surface density of objects can not excluded definitively the LBG over-density hypothesis. Moreover we identified three faint objects at less than three arcseconds from \textit{HFLS3} with color consistent with those expected for $z$$\sim$6 galaxies. Deeper data are needed to confirm their redshifts and to study their association with \textit{HFLS3} and the galaxy merger that may be responsible for the massive starburst.Comment: 14 pages, 12 figures, accepted for publication in Ap

Epidemiological, Clinical and Genetic Study of Hypophosphatasia in A Spanish Population: Identification of Two Novel Mutations in The Alpl Gene

Hypophosphatasia (HPP) is a genetic disease caused by one or several mutations in ALPL gene encoding the tissue-nonspecific alkaline phosphatase affecting the mineralization process. Due to its low prevalence and lack of recognition, this metabolic disorder is generally confused with other more frequent bone disorders. An assessment of serum total alkaline phosphatase (ALP) levels was performed in 78,590 subjects. Pyridoxal-5′-phosphate (PLP) concentrations were determined and ALPL gene was sequenced in patients potentially affected by HPP. Functional validation of the novel mutations found was performed using a cell-based assay. Our results showed persistently low serum ALP levels in 0.12% of subjects. Among the studied subjects, 40% presented with HPP-related symptoms. Nine of them (~28%) had a history of fractures, 5 (~16%) subjects showed chondrocalcinosis and 4 (~13%) subjects presented with dental abnormalities. Eleven subjects showed increased PLP concentrations. Seven of them showed ALPL gene mutations (2 of the mutations corresponded to novel genetic variants). In summary, we identified two novel ALPL gene mutations associated with adult HPP. Using this protocol, almost half of the studied patients were diagnosed with HPP. Based on these results, the estimated prevalence of mild HPP in Spain could be up to double than previously reported.Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (with support from NIH P41-GM103311)grants from Alexion and FEIOMM, by Instituto de Salud Carlos III (grants PI18-00803 and PI18-01235)co-funding from FEDER and by Junta de Andalucía (grant PI-0207-2016)GM-N is supported by the predoctoral program from Instituto de Salud Carlos III (FI17/00178) and by the Research Initiation Grants for Official Master Students program from the University of Granada (2017)PJR is a Ramon y Cajal Researcher from the MINECO (RYC-2015-18383) at GENyO and University of Granada

HerMES: Candidate Gravitationally Lensed Galaxies and Lensing Statistics at Submillimeter Wavelengths

We present a list of 13 candidate gravitationally lensed submillimeter galaxies (SMGs) from 95 square degrees of the Herschel Multi-tiered Extragalactic Survey, a surface density of 0.14\pm0.04deg^{-2}. The selected sources have 500um flux densities (S_500) greater than 100mJy. Gravitational lensing is confirmed by follow-up observations in 9 of the 13 systems (70%), and the lensing status of the four remaining sources is undetermined. We also present a supplementary sample of 29 (0.31\pm0.06deg^{-2}) gravitationally lensed SMG candidates with S_500=80--100mJy, which are expected to contain a higher fraction of interlopers than the primary candidates. The number counts of the candidate lensed galaxies are consistent with a simple statistical model of the lensing rate, which uses a foreground matter distribution, the intrinsic SMG number counts, and an assumed SMG redshift distribution. The model predicts that 32--74% of our S_500>100mJy candidates are strongly gravitationally lensed (mu>2), with the brightest sources being the most robust; this is consistent with the observational data. Our statistical model also predicts that, on average, lensed galaxies with S_500=100mJy are magnified by factors of ~9, with apparently brighter galaxies having progressively higher average magnification, due to the shape of the intrinsic number counts. 65% of the sources are expected to have intrinsic 500micron flux densities less than 30mJy. Thus, samples of strongly gravitationally lensed SMGs, such as those presented here, probe below the nominal Herschel detection limit at 500 micron. They are good targets for the detailed study of the physical conditions in distant dusty, star-forming galaxies, due to the lensing magnification, which can lead to spatial resolutions of ~0.01" in the source plane.Comment: ApJ in press. 31 pages, 16 figures, 5 tables. This version updated to match accepted versio

Transcriptional profiling of the ovine abomasal lymph node reveals a role for timing of the immune response in gastrointestinal nematode resistance

Gastrointestinal nematodes are a serious cause of morbidity and mortality in grazing ruminants. The major ovine defence mechanism is acquired immunity, with protective immunity developing over time in response to infection. Nematode resistance varies both within and between breeds and is moderately heritable. A detailed understanding of the genes and mechanisms involved in protective immunity, and the factors that regulate this response, is required to aid both future breeding strategies and the development of effective and sustainable nematode control methods. The aim of this study was to compare the abomasal lymph node transcriptome of resistant and susceptible lambs in order to determine biological processes differentially expressed between resistant and susceptible individuals. Scottish Blackface lambs, with divergent phenotypes for resistance, were challenged with 30,000 Teladorsagia circumcincta larvae (L3), and abomasal lymph nodes recovered at 7 and 14 days post-infection (dpi). High-throughput sequencing of cDNA from the abomasal lymph node was used to quantitatively sample the transcriptome with an average of 32 million reads per sample. A total of 194 and 144 genes were differentially expressed between resistant and susceptible lambs at 7 and 14 dpi respectively. Differentially expressed networks and biological processes were identified using Ingenuity Pathway Analysis. Genes involved in the inflammatory response, attraction of T lymphocytes and binding of leukocytes were more highly expressed in resistant animals at 7 dpi and in susceptible animals at 14 dpi indicating that resistant animals respond to infection earlier than susceptible animals. Twenty-four Single Nucleotide Polymorphisms (SNP) within 11 differentially expressed genes, were tested for association with gastrointestinal nematode resistance in the Scottish Blackface lambs. Four SNP, in 2 genes (SLC30A2 and ALB), were suggestively associated with faecal egg count. In conclusion, a large number of genes were differentially expressed in the abomasal lymph node of resistant and susceptible lambs responding to gastrointestinal nematode challenge. Resistant Scottish Blackface lambs appear to generate an earlier immune response to T. circumcincta. In susceptible lambs this response appears to be delayed. SNP in 2 differentially expressed genes were suggestively associated with faecal egg count indicating that differentially expressed genes may be considered candidate loci for mediating nematode resistance

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion

ADN bacteriano en pacientes con cirrosis y ascitis estéril: Papel como marcador de translocación bacteriana y herramienta pronóstica Bacterial DNA in patients with cirrhosis ans sterile ascites: Its role as a marker of bacterial translocation and prognosis tool

Durante la última década hemos presenciado un aumento de la cantidad de datos relativos a la presencia de translocación bacteriana en los modelos experimentales de cirrosis. Sin embargo, los estudios clínicos se han visto limitados por la falta de métodos no invasivos para estudiar dicho fenómeno. En los últimos años, las investigaciones realizadas en nuestro laboratorio se han centrado en la detección del ADN bacteriano en el suero y el líquido ascítico de los pacientes con cirrosis y ascitis estéril, y en las implicaciones clínicas que ello conlleva. Al principio, gracias a un método basado en la reacción en cadena de la polimerasa (PCR) y el secuenciamiento automatizado de nucleótidos, pudimos detectar e identificar la presencia de fragmentos de ADN bacteriano en dichos pacientes con ascitis no neutrocítica y con cultivo negativo. Desde entonces hemos acumulado una serie de datos que indican que la presencia de ADN bacteriano podría desempeñar un papel importante no sólo como marcador de translocación bacteriana, sino también como factor pronóstico a corto plazo. Expondremos aquí el pasado, el presente y el futuro de esta línea de investigación.During the last decade, we have witnessed an increase in the amount of data related with the presence of bacterial translocation in experimental models of cirrhosis. However, clinical studies have been limited by the lack of non-invasive methods to study this phenomenon. Over the past years, the research developed in our laboratory has been focused on the detection of bacterial DNA in serum and ascitic fluid of patients with cirrhosis and sterile ascites, the clinical and immunological implications of such finding. Initially, by means of a polymerase chain reaction (PCR)-based method and automated nucleotide sequencing, we were able to detect and identify the presence of fragments of bacterial DNA in the mentioned patients with culture-negative, non-neutrocytic ascites. Since then, we have accumulated a core of data suggesting that the presence of bacterial DNA may have an important role not only as a marker of bacterial translocation, but also as a short-term prognostic factor. Here, we discuss the past, present and future of this line of investigation

Análisis sensorial descriptivo de vinos tintos crianza riojanos: valoración de los críticos de vinos

Póster presentado en el XII Congreso de los Grupos de Investigación Enológica (GIENOL 2013, Nuevas perspectivas en investigación vitivinícola), celebrado en Madrid del 18 al 21 de junio de 2013.Peer Reviewe